Get tips on using Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) to perform ChIP Anti-bodies H3K27me3
Get tips on using Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) to perform ChIP Anti-bodies H3K27ac
Get tips on using Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (ab14748) to perform Western blotting ATP5A
Get tips on using Recombinant Anti-HDAC1 antibody [EPR460(2)] (ab109411) to perform Western blotting HDAC1
Get tips on using Anti-STAT1 (phospho Y701) antibody [M135] (ab29045) to perform Western blotting STAT1
Get tips on using Anti-Mucin 5AC antibody [2-11M1] (ab24071) to perform Western blotting MUC5AC
In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.
In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.
Get tips on using Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441) to perform ChIP Anti-bodies H3K9-Ac
Get tips on using Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) to perform Western blotting tubulin gamma
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