Microarray Gene expression arrays Rat chorid plexus

Get tips on using FlashTag™ Biotin HSR RNA Labeling Kits to perform Microarray RNA amplification & Labeling - Mouse skin tissue Biotin

Get tips on using FlashTag™ Biotin HSR RNA Labeling Kits to perform Microarray RNA amplification & Labeling - Mouse mammary tissue Biotin

Get tips on using miRCURY LNA™ microRNA Power Labeling Kits to perform Microarray RNA amplification & Labeling - HUVEC Hy3 and Hy5

Get tips on using miRCURY LNA™ microRNA Power Labeling Kits to perform Microarray RNA amplification & Labeling - LNCaP Hy3 and Hy5

Get tips on using Stealth siRNA(r)_Ctnnb1 to perform siRNA / miRNA gene silencing Rat - NPC β-Catenin/Ctnnb1

Get tips on using Silencer® Select_Vamp2 siRNA(r) to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Vamp2

Get tips on using Silencer® Select_Vamp7 siRNA(r) to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Vamp7

Get tips on using IGF-IRα/β siRNA (r) to perform siRNA / miRNA gene silencing Rat - RGC-5 IGF1R

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Hello Iam a phd student in pharmacy and i want to know if this technology is suitable to knockout or silencing part of the gas5 gene in BV2 cells please