Protein Expression Eukaryotic cells N. benthamiana

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - 3T3-L1

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - THP-1

Get tips on using Trichloroacetic acid to perform Protein isolation Mammalian cells - THP-1

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - CHO-K1

Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Huh7

Get tips on using NP40 Cell Lysis Buffer to perform Protein isolation Mammalian cells - BHK-21

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - Caco-2

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - SH-SY5Y

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - BHK-21

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase