siRNA / miRNA gene silencing Mouse B16 Melanoma cells

Get tips on using GATA-1 shRNA Plasmids (h) to perform shRNA gene silencing Human - TF‐1 GATA‐1

Reporter gene assays are designed to test the regulation of the expression of a gene of interest. This is usually done by linking the promoter of the gene of interest with a gene such as a firefly luciferase, which can be easily detected by addition of luciferin that leads to an enzymatic reaction to produce luminescence. The enzymatic reaction can be correlated to the expression of the gene of interest. Another luciferase gene that can be used is Renilla luciferase. For an appropriate luciferase assay: 1. the reporter should express uniformly in all cells, 2. specifically respond to effectors that the assay intends to monitor, 3. have low intrinsic stability to quickly reflect transcriptional dynamics. It is important to have an equal number of cells plated in each testing condition to avoid any incorrect readouts. Reporter assays could be single or dual reporter assays. The reporter could be both luciferases. Most dual-luciferase assays involve adding two reagents to each sample and measuring luminescence following each addition. Adding the first reagent activates the first luciferase reporter reaction; adding the second reagent extinguishes first luciferase reporter activity and initiates the second luciferase reaction. Dual-luciferase assays have some advantages, including 1. reduces variability, 2. reduces background, 3. normalizes differences in transfection efficiencies between samples.

Get tips on using Purified Rat Anti-Mouse CD31 to perform Immunohistochemistry Mouse - CD31

Get tips on using Biotin Rat Anti-Mouse CD24 to perform Immunohistochemistry Mouse - CD24

Get tips on using Mouse/Rat Neuropilin-1 Antibody to perform Immunohistochemistry Mouse - Nrp1

Get tips on using Mouse VEGF ELISA Kit (ab100751) to perform ELISA Mouse - VEGF

Get tips on using Mouse VEGF Quantikine ELISA Kit to perform ELISA Mouse - VEGF

Get tips on using Mouse Shh-N ELISA Kit to perform ELISA Mouse - ShhN

Get tips on using RBP4 ELISA Kit (Mouse) (OKBB00339) to perform ELISA Mouse - RBP4

Get tips on using Mouse RBP4 Quantikine ELISA Kit to perform ELISA Mouse - RBP4