DNA methylation profiling Gene specific profiling HepG2

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DNA isolation / purification Cells - Immortalized cell lines Human Neuroblastoma Cell Lines Experiment

DNA DNA isolation / purification Cells Immortalized cell lines Human Neuroblastoma Cell Lines

siRNA / miRNA gene silencing Human - HNSCC cell line Eph receptor B4 Polymer / Lipid Experiment

RNA siRNA / miRNA gene silencing Human HNSCC cell line Eph receptor B4 Polymer / Lipid

siRNA / miRNA gene silencing Human - Primary Human Aortic Endothelial Cells GLO-1 Lipid Experiment

RNA siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1 Lipid

siRNA / miRNA gene silencing Human - 501 Mel and SK Mel 28 FANCD2 Polymer / Lipid Experiment

RNA siRNA / miRNA gene silencing Human 501 Mel and SK Mel 28 FANCD2 Polymer / Lipid

siRNA / miRNA gene silencing Human - Primary Endometrial Stromal Cells hsa-miR-542-3p Lipid Experiment

RNA siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p Lipid

Host Cell Residual DNA contamination LANCE Ultra TR-FRET Detection Kit, 500 Assay Points Product

Get tips on using Host Cell Residual DNA contamination LANCE Ultra TR-FRET Detection Kit, 500 Assay Points to perform Cell Culture Contamination Detection Kit Bacteria

Products PerkinElmer Host Cell Residual DNA contamination LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

PureLink™ HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA Product

Get tips on using PureLink™ HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA to perform Plasmid Isolation Citrobacter koseri

Products Thermo Fisher Scientific PureLink™ HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA

300 prep FavorPrep™ Plasmid DNA Extraction Mini Kit (sample size: 1~ 5 ml culture cells) Product

Get tips on using 300 prep FavorPrep™ Plasmid DNA Extraction Mini Kit (sample size: 1~ 5 ml culture cells) to perform Plasmid Isolation Vibrio parahaemolyticus

Products Favorgen 300 prep FavorPrep™ Plasmid DNA Extraction Mini Kit (sample size: 1~ 5 ml culture cells)

siRNA/RNAi/miRNA transfection human Discussion

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Discussions siRNA/RNAi/miRNA transfection human

siRNA / miRNA gene silencing Human - Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1) Lipid Experiment

RNA siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1) Lipid

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