Flow cytometry Anti-bodies Human

Get tips on using ON-TARGETplus Human PCSK6 (5046) siRNA - Individual to perform siRNA / miRNA gene silencing Human - A253 PACE4

Get tips on using ON-TARGETplus Human FURIN (5045) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A253 Furin

Get tips on using SurePrint G3 Human CGH Microarray Kit, 4x180K to perform Microarray Comperative genomic hybridization - Human Blood cells

Get tips on using SurePrint G3 Human CGH Microarray Kit, 2x400K to perform Microarray Comperative genomic hybridization - Human Blood cells

Get tips on using SurePrint G3 Human CGH Microarray Kit, 4x180K to perform Microarray Comperative genomic hybridization - Human SH-SY5Y

Get tips on using SurePrint G3 Human CGH Microarray Kit, 2x400K to perform Microarray Comperative genomic hybridization - Human U-251

Get tips on using SurePrint G3 Human CGH Microarray Kit, 4x180K to perform Microarray Comperative genomic hybridization - Human Bone marrow

Get tips on using Human Candida Albicans ELISA Kit to perform Cell Culture Contamination Detection Kit Yeast

Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media PDX mammospheres

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.