siRNA / RNAi /miRNA transfection Human Cells KG-1

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using Dynabeads™ Untouched™ Human B Cells Kit to perform Cell Isolation B cell

Get tips on using Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit to perform ELISA Mouse - KIM-1

Get tips on using Rat Kidney injury molecule 1,Kim-1 ELISA Kit to perform ELISA Rat - KIM-1

Get tips on using Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit to perform ELISA Rat - KIM-1

Get tips on using EMBacY#1 to perform Protein Expression Eukaryotic cells - Hi5 mDicer2

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using INTERFERin® to perform siRNA / miRNA gene silencing Human - 501 Mel and SK Mel 28 FANCD2 Polymer / Lipid

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Nectin 1 Monoclonal Antibody (CK8) to perform Flow cytometry Anti-bodies Human - CD111/Nectin-1