siRNA / RNAi /miRNA transfection Rat

Get tips on using Purified anti-mouse/rat/human FOXP3 Antibody to perform Western blotting FOXP3

Get tips on using Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit to perform ELISA Mouse - OPN

Get tips on using Rat CRP/C Reactive Protein PicoKine™ ELISA Kit to perform ELISA Rat - C-Reactive Protein/CRP

Get tips on using Human/Mouse/Rat/Canine ALCAM/CD166 Antibody to perform Immunohistochemistry Mouse - CD166 / ALCAM

Get tips on using Rat/Mouse Cytochrome c Quantikine ELISA Kit to perform ELISA Mouse - Cytochrome c

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Anti Type X Collagen (raised against rat) pAb (Rabbit, Antiserum) to perform Immunohistochemistry Mouse - Col X

Get tips on using BioMag Goat Anti-Rat IgG (500 ml) to perform Cell Isolation Mouse T cells

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.