siRNA / miRNA gene silencing Mouse P19

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using NeuroCult™ Basal Medium (Mouse & Rat) to perform 3D Cell Culture Media Mouse embryonic neurospheres

Get tips on using Purified anti-mouse TNF-α Antibody to perform Flow cytometry Anti-bodies Mouse - TNF-α

Get tips on using FITC anti-mouse CD95 (Fas) Antibody to perform Flow cytometry Anti-bodies Mouse - CD95/Fas

Get tips on using APC anti-mouse CD252 (OX40L) Antibody to perform Flow cytometry Anti-bodies Mouse - CD252/OX40L

Get tips on using Purified Rat Anti-Mouse IFN-γ to perform Flow cytometry Anti-bodies Mouse - IFN-γ

Get tips on using APC Rat Anti-Mouse IFN-γ to perform Flow cytometry Anti-bodies Mouse - IFN-γ

Get tips on using Purified Rat Anti-Mouse Siglec-F to perform Flow cytometry Anti-bodies Mouse - Siglec F

Get tips on using BV421 Rat Anti-Mouse Siglec-F to perform Flow cytometry Anti-bodies Mouse - Siglec F

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.