Bacterial cell culture media

Get tips on using EvaGreen® Dye to perform PCR Quantitative real-time PCR - Bacterial DNA

Get tips on using Mastermix 16S Complete to perform PCR Quantitative real-time PCR - Bacterial DNA

Get tips on using HotStarTaq DNA Polymerase (25000) to perform PCR Hot start PCR - Bacterial DNA

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Hot start PCR - Bacterial DNA

Get tips on using Promega PCR Master Mix to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using GoTaq® DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using QuantiTect Multiplex PCR Kit (1000) to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell Differentiation media iPSCs or hESCs differentiation into cerebellar neuroepithelium (NE)

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.