Protein expression and purification Bacteria Escherichia coli

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - HK-2

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - CAKI-1

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - HEK 293

Get tips on using AllPrep DNA/RNA/Protein Mini Kit to perform Protein isolation Tissue - Human placental tissue

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - MDA-MB-231

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - MDA-MB-231

Get tips on using Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) to perform Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) - Mouse Human -NA-

Get tips on using Micro BCA™ Protein Assay Kit to perform Protein quantification Mammalian cells - Human pluripotent stem cells

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Human pluripotent stem cells

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.