Bacterial cell culture media

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using QuantiFast Multiplex PCR Kit to perform PCR Quantitative real-time PCR - Bacterial DNA

Get tips on using QuantiTect Probe PCR Kit (1000) to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using EZ DNA Methylation-Gold Kit to perform PCR Methylation specific PCR - Bacterial DNA

Get tips on using Platinum™ Taq DNA Polymerase to perform PCR Hot start PCR - Bacterial DNA

Get tips on using SYBR GreenER™ qPCR SuperMix Universal to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using AmpliTaq Gold™ 360 DNA Polymerase to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using ViralSEQ™ Quantitative Sf-Rhabdovirus Kit with PrepSEQ™ Nucleic Acid Sample Prep Kit to perform Cell Culture Contamination Detection Kit Virus

Get tips on using Type-it HRM PCR Kit (2000) to perform High-resolution melting (HRM) analysis Bacterial