siRNA / miRNA gene silencing Human CRL-5915

Get tips on using Human Leptin DuoSet ELISA to perform ELISA Human - Leptin

Get tips on using Human LBP DuoSet ELISA to perform ELISA Human - LBP

Get tips on using Human HSP70 ELISA Kit to perform ELISA Human - HSP70

Get tips on using Human GDNF DuoSet ELISA to perform ELISA Human - GDNF

Get tips on using Human Fibronectin DuoSet ELISA to perform ELISA Human - Fibronectin

Get tips on using Human Decorin ELISA Kit to perform ELISA Human - Decorin

Get tips on using Human Decorin DuoSet ELISA to perform ELISA Human - Decorin

Get tips on using pGL3-Basic Vector to perform Reporter gene assay luciferase - SW480 human colon cancer cell line

Get tips on using Human Melanocyte Media to perform Mammalian cell culture media HEM

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.