siRNA / miRNA gene silencing Rat INS-1

Get tips on using Thyroid Transcription Factor-1 to perform Immunohistochemistry Human - TTF-1

Get tips on using Claudin 1 Polyclonal Antibody to perform Western blotting Cclaudin-1

Get tips on using Human Dkk-1 ELISA to perform ELISA Human - Dkk-1

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using HIF-1 alpha Antibody to perform Western blotting HIF-1 alpha

Get tips on using PE-Cy™7 Rat Anti-Mouse TNF to perform Flow cytometry Anti-bodies Mouse - TNF-α

Get tips on using Alexa Fluor® 488 Rat Anti-Mouse CD146 to perform Flow cytometry Anti-bodies Mouse - CD146/MCAM

Get tips on using mirVana® miRNA mimic to perform RNA isolation / purification Cells - primary human endothelial cells

Get tips on using SurePrint Human miRNA Microarrays to perform Microarray Human - Endometrial stromal cells miRNA-expression array (labelled)