DNA isolation / purification Bacteria Gram negative

Get tips on using BenchTop 100bp DNA Ladder to perform DNA Ladder 100 bp

Get tips on using 1kb DNA Step Ladder to perform DNA Ladder 1 kb

Get tips on using 100bp DNA Step Ladder to perform DNA Ladder 100 bp

Get tips on using 100 bp DNA Ladder to perform DNA Ladder 100 bp

Get tips on using 123 bp DNA Ladder to perform DNA Ladder 123 bp

Get tips on using 200bp DNA Step Ladder to perform DNA Ladder 200 bp

Get tips on using BenchTop 1kb DNA Ladder to perform DNA Ladder 1 kb

Get tips on using 1 kb DNA Ladder to perform DNA Ladder 1 kb

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.