DNA methylation profiling Gene specific profiling MCF-7

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Monkey - Point mutation Cos-7 GARS

Get tips on using Q5® Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Monkey - Deletion COS-7 PNPLA7

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human RT-7

Get tips on using eBioscience™ Annexin V-FITC Apoptosis Detection Kit to perform Apoptosis assay cell type - Caspase 3/7

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Human - MCF10A

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - MCF10A

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification MCF10A breast epithelial cells