Immunohistochemistry CD31 Rabbit Rat

Get tips on using APC Mouse Anti-Human CD71 to perform Flow cytometry Anti-bodies Human - CD71

Get tips on using Individual: TRC Mouse Cdh1 shRNA to perform shRNA gene silencing Mouse - 4T1 Cdh1

Get tips on using CD61-FITC, SZ21, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD61

Get tips on using CD41-PE, P2, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD41

Get tips on using APC-H7 Mouse Anti-Human CD71 to perform Flow cytometry Anti-bodies Human - CD71

Get tips on using ON-TARGETplus Human CDK1 (983) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 CDK1

Get tips on using PE Mouse Anti-Human CD61 Clone VI-PL2 to perform Flow cytometry Anti-bodies Human - CD61

Get tips on using PE-Cy™7 Mouse Anti-Human CD41a to perform Flow cytometry Anti-bodies Human - CD41

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.