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|- Prepare 1x Binding Buffer by diluting 1 ml of the 10x Binding Buffer with 9 ml of deionized water.
- Dissolve the staurosporine in DMSO to a concentration of 100 mg/ml.
- cells were pretreated with BER for 1 h, then cultured with or without fMLP (1µM) stimulation for 12 h
|- Apoptotic neutrophils are here defined as Annexin V-positive but propidium iodide-negative cells.|
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