rna-isolation-purification-tissue-mouse-colon

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using VWR Life Science RiboZol™ RNA Extraction Reagent to perform RNA isolation / purification Cells - immortalized C2C12

Get tips on using Quick-RNA Microprep Kit to perform RNA isolation / purification Cells - primary human pulmonary artery endothelial cells

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - primary human renal proximal tubular epithelial cells

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - primary human endometrial stromal cells

Get tips on using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents to perform Protein isolation Tissue - Mouse skeletal muscle

Get tips on using E.Z.N.A.® Total RNA Kit I to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus epidermidis

Get tips on using ChargeSwitch™ Total RNA Cell Kit to perform RNA isolation / purification Bacteria - Gram negative Hemophilus influenzae

Get tips on using ChargeSwitch™ Total RNA Cell Kit to perform RNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

Get tips on using Direct-zol™ RNA MiniPrep Plus to perform RNA isolation / purification Bacteria - Gram negative Borrelia burgdorferi