siRNA / miRNA gene silencing Human THP-1

Get tips on using Rat IGF-1 ELISA Kit (ab213902) to perform ELISA Rat - IGF-I

Get tips on using Anti-HIF-1 alpha antibody - ChIP Grade (ab2185) to perform Western blotting HIF-1 alpha

Get tips on using Mouse TGF Beta 1 PicoKine™ ELISA Kit to perform ELISA Mouse - TGF-beta 1

Get tips on using Mouse SDF-1 α / CXCL12 α ELISA Kit to perform ELISA Mouse - SDF-1/CXCL12

Get tips on using Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit to perform ELISA Mouse - SDF-1/CXCL12

Get tips on using Mouse IL-1 Beta PicoKine™ ELISA Kit to perform ELISA Mouse - IL-1 beta

Get tips on using Mouse ICAM-1 / CD54 PicoKine™ ELISA Kit to perform ELISA Mouse - ICAM-1/CD54

Get tips on using Rat TGF Beta 1 PicoKine™ ELISA Kit to perform ELISA Rat - TGF-beta 1

Get tips on using Rat IL-1 Beta PicoKine™ ELISA Kit to perform ELISA Rat - IL-1 beta

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.