CRISPR Mouse Deletion ES (embryonic stem) cells

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media mPSCs/mESCs differentiation to Embryoid body (EB)

Get tips on using Gibco™Glasgow's MEM (GMEM) to perform Stem cell Differentiation media mPSCs/mESCs differentiation to Embryoid body (EB)

Get tips on using Gibco™KnockOut™ DMEM to perform Stem cell Differentiation media mPSCs/mESCs differentiation to Embryoid body (EB)

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using StemSpan™ SFEM II to perform Stem cell culture media hHSCs

Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation interferon-γ promoter

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Activation PAX5

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Activation RANKL

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Repression PrPC

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Repression EZH2