DNA transfection Mammalian cells Primary cells

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Get tips on using Gibco DMEM/F-12, HEPES to perform 3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids

Products Thermo Fisher Scientific Gibco DMEM/F-12, HEPES

Cellular assays Cell Isolation hematopoietic stem cells

Cellular assays Cell Isolation CD4+ T cells

Cellular assays Cell Isolation Human T cells

Cellular assays Cell Isolation Mouse T cells

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human epidermal melanocytes

Products Thermo Fisher Scientific mirVana™ miRNA Isolation Kit, with phenol

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human epidermal keratinocytes

Products Thermo Fisher Scientific mirVana™ miRNA Isolation Kit, with phenol

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human chondrocytes - osteoarthritis

Products Thermo Fisher Scientific mirVana™ miRNA Isolation Kit, with phenol

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Apoptosis assay cell type T-cells Mouse (OT-I)

Get tips on using Cell Counting Kit-8 to perform Live / Dead assay mammalian cells - GH3

Products Dojindo Cell Counting Kit-8

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