Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Hypothalamus mouse tissue MeCP2
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Mammalian cells - Rat_Liver
Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Mammalian cells - Huh7
Get tips on using DNeasy UltraClean Microbial Kit to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas
Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Human ovarian tissue TGFBI
Get tips on using DNeasy Plant Pro Kit (250) to perform DNA isolation / purification Plants - Mycelial mats
Get tips on using DNeasy PowerClean Pro Cleanup Kit (50) to perform DNA isolation / purification Water samples
Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Mammalian cells - Rat_Mesenteric fat
Get tips on using miRNeasy Serum/Plasma Advanced Kit (50) to perform RNA isolation / purification Tissue - Livestock Blood / Serum / Plasma / Buffy coat
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