siRNA / RNAi /miRNA transfection Human Lung Adenocarcenoma (A549/LTEP-a-2)

Get tips on using CometChip Electrophoresis Starter Kit to perform DNA Damage Assay A549

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized A549

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized A549

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized A549

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized A549

Get tips on using Recombinant Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005) to perform Western blotting Jak1

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized A549

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using PrepSEQ™ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit to perform Cell Culture Contamination Detection Kit Mycoplasma

Get tips on using APG5 Antibody (C-1) to perform Autophagy assay cell type - A549