DNA transfection Mammalian cells Primary cells

Get tips on using EZ4U - Cell Proliferation Assay to perform Live / Dead assay mammalian cells - GH3

Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - HepG2

Get tips on using Mammalian beta-Galactosidase Assay Kit to perform Reporter gene assay β-galactosidase substrates - H9C2

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Human lung fibroblasts

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Mouse Epididymal fat

Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Rat_Renal tissue

Get tips on using TRI Reagent® to perform Protein isolation Mammalian cells - Mouse_Brown fat

Get tips on using RIPA Lysis Buffer, 10X to perform Protein isolation Mammalian cells - HaCaT