Get tips on using Beclin 1 Antibody to perform Autophagy assay cell type - A2780
Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HaCaT
Get tips on using Beclin 1 Rabbit Polyclonal antibody to perform Autophagy assay cell type - A375
Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HepG2
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using Fluorescent 1 kb DNA Ladder to perform DNA Ladder 1 kb
Get tips on using 1 kb Plus DNA Ladder to perform DNA Ladder 1 kb
Get tips on using Human ICAM-1/CD54 Antibody to perform Western blotting ICAM-1
Get tips on using Mouse KIM-1 ELISA Kit to perform ELISA Mouse - KIM-1
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