siRNA / miRNA gene silencing Mouse mESC

Get tips on using PCNA (PC10) Mouse mAb #2586 to perform Western blotting PCNA

Get tips on using Mouse Rat Estradiol ELISA Kit to perform ELISA Rat - Estradiol

Get tips on using Mouse GDNF ELISA Kit (ab171178) to perform ELISA Human - GDNF

Get tips on using Human/Mouse BDNF DuoSet ELISA to perform ELISA Human - BDNF

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Human/Mouse/Rat/Canine ALCAM/CD166 Antibody to perform Immunohistochemistry Mouse - CD166 / ALCAM

Get tips on using Mouse TNFSF11/RANKL PicoKine™ ELISA Kit to perform ELISA Mouse - RANK L

Get tips on using Mouse TNF Alpha PicoKine™ ELISA Kit to perform ELISA Mouse - TNF-alpha

Get tips on using Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083) to perform ELISA Mouse - NGAL/LCN2

Get tips on using Mouse KIM1 ELISA Kit (TIM-1) (ab119596) to perform ELISA Mouse - KIM-1