siRNA / miRNA gene silencing Rat INS-1

Get tips on using Endothelin-1 Quantikine ELISA Kit to perform ELISA Mouse - Endothelin 1

Get tips on using Endothelin 1 ELISA Kit (ab133030) to perform ELISA Mouse - Endothelin 1

Get tips on using Mouse Dkk-1 ELISA Kit to perform ELISA Mouse - Dkk-1

Get tips on using KIM-1 (human) ELISA kit to perform ELISA Human - KIM-1

Get tips on using HO-1 (human), ELISA kit to perform ELISA Human - HO-1

Get tips on using Beclin-1 Antibody to perform Autophagy assay cell type - NRK-49F

Get tips on using Beclin-1 Antibody to perform Autophagy assay cell type - BRL-3A

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Deletion 3T3-L1 Epac1

Get tips on using DIA-310: Anti-CD31 (Ms) from Rat (Clone: SZ31) for mouse FFPE tissue to perform Immunohistochemistry CD31 - Rabbit Rat -NA-

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.