protein-expression-and-purification-mammalian-cells-hek-293-mt-pa

Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Human Cornea

Get tips on using FitAmp Blood and Cultured Cell DNA Extraction Kit to perform DNA isolation / purification Cells - Immortalized cell lines SH-SY5Y

Get tips on using RPMI 1640, with L-Glutamine and 25mM HEPES to perform Mammalian cell culture media DU145

Get tips on using RPMI 1640, with L-Glutamine and 25mM HEPES to perform Mammalian cell culture media VCaP

The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.

Get tips on using One-step TUNEL Assay Kit to perform DNA Damage Assay HEK 293T

Get tips on using Mammalian beta-Galactosidase Assay Kit to perform Reporter gene assay β-galactosidase substrates - H9C2

Get tips on using RPMI 1640, with L-Glutamine and 25mM HEPES to perform Mammalian cell culture media THP-1

Get tips on using RPMI 1640, with L-Glutamine and 25mM HEPES to perform Mammalian cell culture media PC-3

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.