ChIP acH3 Sheep Horse

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Get tips on using ChIP-IT® Express Shearing Kits to perform ChIP Human - SW480

Get tips on using LowCell# ChIP kit protein A x16 to perform ChIP Human - HepG2

Get tips on using EpiQuik Acetyl-Histone H4 ChIP Kit to perform ChIP Human - HepG2

Get tips on using Anti-Rad51 antibody - ChIP Grade (ab176458) to perform ChIP Anti-bodies RAD51

Get tips on using Anti-Mre11 antibody - ChIP Grade (ab12159) to perform ChIP Anti-bodies MRE11

Get tips on using Anti-CTCF antibody - ChIP Grade (ab70303) to perform ChIP Anti-bodies CTCF

Get tips on using Anti-Sall4 antibody - ChIP Grade (ab29112) to perform ChIP Anti-bodies Sall4

Get tips on using Anti-HDAC1 antibody - ChIP Grade (ab7028) to perform ChIP Anti-bodies HDAC1

Get tips on using Magna ChIP™ G Tissue Kit to perform ChIP Mouse - Cardiac fibroblasts