Site Directed Mutagenesis (SDM) Mouse Point mutation C2C12

- Found 5635 results

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Get tips on using Cdx2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - mESC Cdx2

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Get tips on using BECN1 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - BV2 BECN1

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Get tips on using CD98 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - Colon26 CD98

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Get tips on using CD98 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - RAW264.7 CD98

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Get tips on using Mm_Hdac5_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 HDAC5

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Cell cytotoxicity assays measure the ability of certain compounds or chemical mediators to reduce the viability of the cells. The term cell cytotoxicity assay can sometimes be used interchangeably with cell proliferation assay. Healthy living cells can be identified by the use of formazan dyes, protease biomarkers or by measuring ATP content. The formazan dyes are chromogenic products formed by the reduction of tetrazolium salts by dehydrogenases, such as lactate dehydrogenase (LDH) and reductases that are released during cell death. Common tetrazolium salts include INT, MTT, MTS and XTT. Cell cytotoxicity can also be measured by using the SRB and WST-1 assays. These assays can usually be used in a high-throughput fashion and can be quantitated by measuring absorbance, colorimetry or luminescence. All these assays require similar numbers of cell plating at the initiation, a time course of treatment with the cytotoxic agent and at least triplicates for each condition at every point of analysis. Cell shrinkage, plasma membrane blebbing, cell detachment, externalization of phosphatidylserine, nuclear condensation and ultimately DNA fragmentation are well-described features of apoptosis. The assays that rely on cell membrane integrity for their function, may not be able to quantify early apoptosis. Therefore, in order to distinguish early apoptotic vs. late apoptotic or necrotic cells, additional flow cytometry techniques can be used. A combination of Annexin V and PI (propidium iodide) can be used to distinguish early (Annexin V+/PI-) and late apoptotic (Annexin V+/PI+) cells. Sometimes, caspase assays are used in order to differentiate the stages of apoptosis.

Cellular assays Cell cytotoxicity / Proliferation assay cell type adipose stem cells
MLM3636 Product

Get tips on using MLM3636 to perform CRISPR Mouse - Deletion Neuro 2a Prnp

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JDS246 Product

Get tips on using JDS246 to perform CRISPR Mouse - Deletion Neuro 2a Prnp

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Get tips on using lentiCRISPR to perform CRISPR Mouse - Deletion RAW 264.7 Tfeb

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