Site Directed Mutagenesis (SDM) Human Point mutation U2OS

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DNA DNA isolation / purification Micorbiome Human gut

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells bmMSCs human bone marrow

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells SH-SY5Y Human neuroblastoma

Get tips on using CD163 Antibody, anti-human, PE-Vio® 770, REAfinity™ to perform Flow cytometry Anti-bodies Human - CD163

Products Miltenyibiotec CD163 Antibody, anti-human, PE-Vio® 770, REAfinity™

Get tips on using Alexa Fluor® 488 anti-human CD127 (IL-7Rα) Antibody to perform Flow cytometry Anti-bodies Human - CD127

Products BioLegend Alexa Fluor® 488 anti-human CD127 (IL-7Rα) Antibody

Get tips on using Alexa Fluor® 488 anti-human CD15 (SSEA-1) Antibody to perform Flow cytometry Anti-bodies Human - CD15

Products BioLegend Alexa Fluor® 488 anti-human CD15 (SSEA-1) Antibody

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase human embryonic stem cells

Get tips on using EasySep™ Human CD33 Positive Selection Kit II to perform Cell Isolation Monocyte

Products STEMCELL technologies EasySep™ Human CD33 Positive Selection Kit II

Get tips on using Monoclonal Mouse Anti-Human Hepatocyte (Concentrate) Clone OCH1E5 to perform Immunohistochemistry Mouse - Hepatocyte

Products Agilent Technologies Monoclonal Mouse Anti-Human Hepatocyte (Concentrate) Clone OCH1E5

Get tips on using Human/Mouse/Rat Activin A Quantikine ELISA Kit to perform ELISA Rat - Activin

Products R&D Systems Human/Mouse/Rat Activin A Quantikine ELISA Kit

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