Live / Dead assay mammalian cells - SH-SY5Y Human neuroblastoma

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

LIVE/DEAD™ Cell Imaging Kit

Thermo Fisher Scientific

Upstream tips
The stains do not survive fixation or permeabilization.
Protocol tips
Incubate cells at 37 °C for 4 h
Downstream tips
Image quality may be improved by replacing media with Live Cell Imaging Solution
Upstream tips
Treat cells with 90 µM of MPTQ in their cell culture medium before adding reagent.
Protocol tips
Incubate cells in dark for 30 minutes at room temperature.
Protocol tips
Add 100 µl of the Calcein-AM/EthD-III to each well.

Incubate the samples at room temperature for 30–45 minutes.
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