ROS assay cell type

Get tips on using 3 µm Chemotaxis Assays, 96-Well Format to perform Cell migration / Invasion cell type - Jurkat

Get tips on using 5 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - A549

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Live / Dead assay mammalian cells - CHO-K1

Get tips on using Qubit™ Protein Assay Kit to perform Protein quantification Mammalian cells - OUMS-27

Get tips on using QuantiPro™ BCA Assay Kit to perform Protein quantification Mammalian cells - HEK 293

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human trophoblast cells

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification MCF10A breast epithelial cells

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Live / Dead assay mammalian cells - mouse bone marrow-derived macrophages