Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 4 matching solutions for this experiment

Protocol tips
Add the MTT Reagent to each well at a 1:10 ratio.

Incubate the wells 2-4 hours or overnight at 37°C.

Add Detergent Solution and incubate in the dark for 2-4 hours at room temperature
Protocol tips
Stain cells with 0.5 ml staining solution containing 0.5 μl Live-Dye (1 mM) and 0.5 μl PI (2.5 mg/ml).

Incubate for 15 min at 37°C
Protocol tips
Add ethidium-calcein mixture, and incubate for 30 min in a 37 °C incubato
LIVE/DEAD™ Cell Imaging Kit

Thermo Fisher Scientific

Protocol tips
Add working solution and incubate for 15–30 min at 37 °C
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