rna-isolation-purification-tissue-mouse-spine

Get tips on using 3D-Gene® Mouse miRNA Oligo chip (ver.21) to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray RNA amplification & Labeling - Mouse brain tissue Biotin

Get tips on using AllPrep Bacterial DNA/RNA/Protein Kit (50) to perform DNA isolation / purification Bacteria - Gram negative Massilia sp

Get tips on using RNeasy PowerBiofilm Kit (50) to perform RNA isolation / purification Biofilm

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Raji

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Raji

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using ISOGEN to perform RNA isolation / purification Cells - immortalized CHO

Get tips on using TIANamp Genomic DNA Kit to perform DNA isolation / purification Cells - Primary cells Mouse embryonic fibroblast (MEF)

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Yeast - Ustilago maydis