rna-isolation-purification-cells-primary-human-carotid-artery-endothelial-cells

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human lung fibroblasts (HLF)

Get tips on using VWR Life Science RiboZol™ RNA Extraction Reagent to perform RNA isolation / purification Tissue - Human Gallbladder

Get tips on using NucleoSpin® RNA Blood to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - rat kidney tissue

Get tips on using PureLink™ FFPE RNA Isolation Kit to perform RNA isolation / purification Tissue - rat kidney tissue

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized Mono-Mac-6

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.