DNA methylation profiling Gene specific profiling Hypothalamus mouse tissue

Get tips on using Mouse MPO ELISA Kit (Myeloperoxidase) (ab155458) to perform ELISA Mouse - MPO

Get tips on using pro-MMP-9 Mouse ELISA Kit to perform ELISA Mouse - MMP9

Get tips on using Mouse/Rat Leptin Quantikine ELISA Kit to perform ELISA Mouse - Leptin

Get tips on using Mouse GDNF PicoKine™ ELISA Kit to perform ELISA Mouse - GDNF

Get tips on using Mouse Fibronectin PicoKine™ ELISA Kit to perform ELISA Mouse - Fibronectin

Get tips on using Mouse Decorin ELISA Kit (DCN) (ab155454) to perform ELISA Mouse - Decorin

Get tips on using Mouse BDNF PicoKine™ ELISA Kit to perform ELISA Mouse - BDNF

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Mouse TNF alpha ELISA Kit (ab208348) to perform ELISA Mouse - TNF-alpha