Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Get tips on using Viromer® RED to perform DNA transfection Mammalian cells - Immortalized cell lines HEK293
Get tips on using Viromer® RED to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa
Get tips on using PolyFect Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines CHO
Get tips on using PolyFect Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines HEK293
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Rat cell lines
Get tips on using GenJet™ In Vitro DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines COS7
Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - Mouse Adipose
Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Immortalized cell lines 3T3
Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Immortalized cell lines HeLa
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