Get tips on using APC-Cy™7 Mouse Anti-Human CD3 to perform Flow cytometry Anti-bodies Human - CD3
Get tips on using Brilliant Violet 570™ anti-human CD27 Antibody to perform Flow cytometry Anti-bodies Human - CD27
Get tips on using Brilliant Violet 605™ anti-human CD69 Antibody to perform Flow cytometry Anti-bodies Human - CD69
Get tips on using PE/Dazzle™ 594 anti-human CD69 Antibody to perform Flow cytometry Anti-bodies Human - CD69
Get tips on using PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα) Antibody to perform Flow cytometry Anti-bodies Human - CD127
Get tips on using Alexa Fluor® 647 Mouse Anti-Human CD24 to perform Flow cytometry Anti-bodies Human - CD24
Get tips on using Human Serpin E1/PAI-1 Quantikine ELISA Kit to perform ELISA Human - Serpin E1/PAI-1
Get tips on using Rabbit Anti-Human CHK2 (NT) Affinity Purified pAb to perform Immunohistochemistry chk2 - Rabbit IgG Human -NA-
Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
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