shRNA gene silencing Human HEK 293T CAPN5- (Calpains)

Get tips on using p62 (human) polyclonal antibody to perform Immunohistochemistry Mouse - p62

Get tips on using Human MMP-1 Antibody to perform Western blotting MMP1

Get tips on using Human GDNF DuoSet ELISA to perform ELISA Rat - GDNF

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Human VEGF PicoKine™ ELISA Kit to perform ELISA Human - VEGF

Get tips on using Human SDC1 / Syndecan-1 ELISA Kit to perform ELISA Human - SDC1

Get tips on using Human Prolactin PicoKine™ ELISA Kit to perform ELISA Human - PRL

Get tips on using Human Osteopontin (OPN) Quantikine ELISA Kit to perform ELISA Human - OPN

Get tips on using Human MCP-1 ELISA Kit (ab179886) to perform ELISA Human - MCP1

Get tips on using Human Myeloperoxidase ELISA Kit (MPO) (ab119605) to perform ELISA Human - MPO