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2 years ago
2 years ago by Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
Found 5 matching solutions for this experiment
|If stored at –20°C, 20X SSC must be warmed to room temperature to ensure that all salts are in solution prior to 10X dilution with deionized H2O.
After opening the kit, 20X SSC can be stored at room temperature.
|You must separate Coplin jars that have been used with DNase from DNase-free Coplin jars.|
|- 2.5 × 10^4 cells per well in 24 well plate were seeded.|
|- Cells were stained with anti-TDP-43 (1:150), anti-GFP (1:1000), Alexa 647 anti-rabbit (1:1000) and Alexa 488 anti-chicken (1:1000).
- The enzyme solution (terminal transferase) and label solution were mixed in a volume ratio of 1:9