Stabilization of DNA

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay COV362

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay COV362

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay A549

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Primary cells Mouse embryonic fibroblast (MEF)

Get tips on using DNeasy Blood and Tissue Kit (250) to perform DNA isolation / purification Cells - Immortalized cell lines Loucy

Reporter gene assays are designed to test the regulation of the expression of a gene of interest. This is usually done by linking the promoter of the gene of interest with a gene such as a firefly luciferase, which can be easily detected by addition of luciferin that leads to an enzymatic reaction to produce luminescence. The enzymatic reaction can be correlated to the expression of the gene of interest. Another luciferase gene that can be used is Renilla luciferase. For an appropriate luciferase assay: 1. the reporter should express uniformly in all cells, 2. specifically respond to effectors that the assay intends to monitor, 3. have low intrinsic stability to quickly reflect transcriptional dynamics. It is important to have an equal number of cells plated in each testing condition to avoid any incorrect readouts. Reporter assays could be single or dual reporter assays. The reporter could be both luciferases. Most dual-luciferase assays involve adding two reagents to each sample and measuring luminescence following each addition. Adding the first reagent activates the first luciferase reporter reaction; adding the second reagent extinguishes first luciferase reporter activity and initiates the second luciferase reaction. Dual-luciferase assays have some advantages, including 1. reduces variability, 2. reduces background, 3. normalizes differences in transfection efficiencies between samples.

Get tips on using Hoechst 33258 to perform DNA quantification Human - MDA-MB-231

Get tips on using Sodium bisulfite to perform PCR Methylation specific PCR - Bacterial DNA

Get tips on using HaeIII Methyltransferase to perform PCR Methylation specific PCR - Bacterial DNA

Get tips on using KAPA LongRange to perform PCR Hot start PCR - Mammalian DNA