siRNA / miRNA gene silencing Human MDA-MB-453

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Get tips on using Human Decorin DuoSet ELISA to perform ELISA Human - Decorin

Products R&D Systems Human Decorin DuoSet ELISA

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Cellular assays ROS assay cell type A549 human adenocarcinomic human alveolar basal epithelial cells

Get tips on using Human FGF-10 Antibody to perform Immunohistochemistry Human - FGF-10

Products R&D Systems Human FGF-10 Antibody

Get tips on using Human Dkk-1 ELISA to perform ELISA Human - Dkk-1

Products Raybiotech Human Dkk-1 ELISA

Get tips on using SurePrint G3 Human CGH Microarray Kit, 4x180K to perform Microarray Comperative genomic hybridization - Human Bone marrow

Products Agilent Technologies SurePrint G3 Human CGH Microarray Kit, 4x180K

Get tips on using psiCHECK-2vector to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells

Products Promega psiCHECK-2vector

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray RNA amplification & Labeling Human endometrial stromal cells Cyanine 3-pCp

Get tips on using ElectraSense 4x2K/12K to perform Microarray Gene expression arrays - Human whole blood cells Biotin

Products Custom Array ElectraSense 4x2K/12K

Cellular assays Cell line authentication Human iPSC cells derived from human dermal fibroblasts

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Cellular assays Wound healing assay cell type human gHMVEC (glioma human microvascular endothelial cells)

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