Site Directed Mutagenesis (SDM) Human Point mutation THP-1

Get tips on using Human GDNF DuoSet ELISA to perform ELISA Rat - GDNF

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Human Apoptosis Array G1 to perform Apoptosis assay cell type - PC-3

Get tips on using Human VEGF PicoKine™ ELISA Kit to perform ELISA Human - VEGF

Get tips on using Human Sonic Hedgehog ELISA Kit (ab100639) to perform ELISA Human - ShhN

Get tips on using Human Prolactin PicoKine™ ELISA Kit to perform ELISA Human - PRL

Get tips on using Human Osteopontin (OPN) Quantikine ELISA Kit to perform ELISA Human - OPN

Get tips on using Human Myeloperoxidase ELISA Kit (MPO) (ab119605) to perform ELISA Human - MPO

Get tips on using Human MMP-9 Quantikine ELISA Kit to perform ELISA Human - MMP9

Get tips on using Human MMP9 ELISA Kit (lyophilized) (ab100610) to perform ELISA Human - MMP9