ELISA

Get tips on using Human IGF1 ELISA Kit (ab100545) to perform ELISA Human - IGF-I

Get tips on using HO-1 (human), ELISA kit to perform ELISA Human - HO-1

Get tips on using GM-CSF Human ELISA Kit to perform ELISA Human - GM-CSF

Get tips on using Human GM-CSF DuoSet ELISA to perform ELISA Human - GM-CSF

Get tips on using Cytochrome C Human ELISA Kit to perform ELISA Human - Cytochrome C

Get tips on using Human BMP2 ELISA Kit (ab119581) to perform ELISA Human - BMP-2

Get tips on using BMP-2 Quantikine ELISA Kit to perform ELISA Human - BMP-2

Get tips on using Mouse SDF1 ELISA Kit (ab100741) to perform ELISA Mouse - SDF-1/CXCL12

Get tips on using CRP (Human) ELISA Kit (KA0238) to perform ELISA Human - C-Reactive Protein/CRP

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.