Flowcytometry CD3 Mouse / IgG1, kappa

Get tips on using APC Mouse Anti-Human CD184 to perform Flow cytometry Anti-bodies Human - CD184/CXCR4

Get tips on using Mouse Osteopontin/OPN Antibody to perform Immunohistochemistry Mouse - Spp1/OPN

Get tips on using BUV395 Mouse Anti-Human CD123 to perform Flow cytometry Anti-bodies Human - CD123/IL3-R

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using Mouse ICAM-1 / CD54 PicoKine™ ELISA Kit to perform ELISA Mouse - ICAM-1/CD54

Get tips on using siGENOME Mouse Alox12 siRNA to perform siRNA / miRNA gene silencing Mouse - B16-F10 12-Lox/ALOX12

Get tips on using Alexa Fluor® 647 Rat Anti-Mouse CD206 to perform Flow cytometry Anti-bodies Mouse - CD206

Get tips on using PE anti-mouse CD49b (pan-NK cells) Antibody to perform Flow cytometry Anti-bodies Mouse - CD49b

Get tips on using PE-Cy™7 Rat Anti-Mouse CD86 to perform Flow cytometry Anti-bodies Mouse - CD86