DNA isolation / purification Bacteria Gram positive

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Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Primary cells Rat dermal fibroblasts (rDF)

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Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines Chang Liver cells

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Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines MDA-MB-231

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Get tips on using Imprint® Methylated DNA Quantification Kit to perform DNA methylation profiling Whole genome profiling - C2C12 mouse myoblast cells

Products Sigma-Aldrich Imprint® Methylated DNA Quantification Kit

Get tips on using MethylFlash™ Methylated DNA Quantification Kit to perform DNA methylation profiling Whole genome profiling - rat renal cortex tissue

Products Epigentek MethylFlash™ Methylated DNA Quantification Kit

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Primary cells Rat mesenchymal stem cells (rMSC)

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Get tips on using Imprint® Methylated DNA Quantification Kit to perform DNA methylation profiling Gene specific profiling - Mouse muscle stem cells SPRY1

Products Sigma-Aldrich Imprint® Methylated DNA Quantification Kit

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Primary cells Rat aortic smooth muscle cells (rASMC)

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Get tips on using AllPrep DNA/RNA Mini Kit to perform AAA for reviews

Products Qiagen AllPrep DNA/RNA Mini Kit

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling HCT116, HTC15 human colon cancer cells

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