rna-isolation-purification-cells-primary-rat-cortical-neurons

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miRNeasy Mini kit Product

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized A2780/DDP

Products Qiagen miRNeasy Mini kit

TruSeq RNA Library Prep Kit v2 Product

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - Retinal ganglion cells (RGCs)

Products Illumina TruSeq RNA Library Prep Kit v2

PolyATtract® mRNA Isolation Systems Product

Get tips on using PolyATtract® mRNA Isolation Systems to perform RNA isolation / purification Yeast - Coprinus cinereus

Products Promega PolyATtract® mRNA Isolation Systems

Some help with RNA isolation using Trizol Discussion

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Discussions Some help with RNA isolation using Trizol

TRI Reagent® Sigma Product

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized DG-75