Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse

Get tips on using ON-TARGETplus Mouse Gpam siRNA to perform siRNA / miRNA gene silencing Mouse - Embryonic stem cells Gpat1

Get tips on using UNveil Unstained Protein Ladder（10 to 200 kDa） to perform Protein Ladder Unstained

Get tips on using Unstained Protein Standard, Broad Range (10-200 kDa) to perform Protein Ladder Unstained

Get tips on using Odyssey® One-Color Protein Molecular Weight Marker to perform Protein Ladder Immunofluorescence

Get tips on using BLUEstain™ 2 Protein ladder, 5-245 kDa to perform Protein Ladder Prestained

Get tips on using IRIS9 Prestained Protein Ladder（15 to 180 kDa） to perform Protein Ladder Prestained

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.